Agricultural and Environmental Biotechnology Abstracts

New research is revealing numerous uses for biotechnological techniques in the food industry, agriculture, and the environment. By the turn of the century, the market for such products and applications is expected to exceed ten billion dollars and this new database gathers the world literature in which molecular biology and genetics are applied to these areas. The database covers agricultural products involving both plants and animals, new applications across the food industry, and wide-ranging environmental developments. Plant genome studies, now the focus of major research initiatives around the world, are examined in detail. Food, chemical, pharmaceutical, and biotechnology companies as well as scientists and other professionals working in agricultural and environmental fields will find information essential to their work in this database.

Subject Coverage

Major areas of coverage include:

• genetic engineering of animals, plants and microorganisms
• plant and animal breeding
• bioinformatics
• methodology
• waste treatment and bioremediation
• fermentation and process engineering
• enzymes and cofactors
• amino acids and proteins
• carbohydrates
• organic acids
• alcohols
• food
• antibodies
• antibiotics and antiviral agents
• vaccines

1993 - current

Monthly, with approximately 300 new records added

Over 54,007 records as of February 2007

Agricultural and Environmental Biotechnology Abstracts (1993 – 2003)

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TI:	Title Integration and expression of Bluetongue VP2 gene in somatic embryos of peanut through particle bombardment method
AU:	Author Athmaram, TN; Bali, Geetha; Devaiah, KM
AF:	Author Affiliation Department of Microbiology and Biotechnology, Bangalore University, Jnanabharathi, Bangalore 560056, India, baliccet@vsnl.com
SO:	Source Vaccine [Vaccine]. Vol. 24, no. 15, pp. 2994-3000. 2006.
IS:	ISSN 0264-410X
DE:	Descriptors Kanamycin; Somatic embryos; Transgenic plants; Nuts; Plantlets; Polymerase chain reaction; VP2 protein; Epitopes; particle bombardment; Reverse transcription; Plasmids; Osmosis; Vaccines; Somatic embryogenesis; Gene transfer; Callus; Coat protein; DNA probes; Bluetongue; Integration; Enzymes; Arachis hypogaea; Bluetongue virus
AB:	Abstract After pre-culture and treatment of osmosis, zygotic embryos of peanut (Arachis hypogaea L.) were transformed via particle bombardment with a plasmid containing a Bluetongue VP2 gene (BTVP2) comprising neutralizing epitopes. Selection for Kanamycin resistant calluses and somatic embryos was initiated at 12th day post-bombardment on medium containing 25 mg/L Kanamycin. Under continuous selection, 12.38% Kanamycin resistant plantlets were regenerated from bombarded somatic embryos. The presence and integration of BTVP2 DNA in regenerated Kanamycin resistant plants were confirmed by southern hybridization assay using non-radioactive Digoxiginin BTVP2 probe. beta - Glucuronidase (GUS) enzyme activity was detected in transgenic somatic embryos but not from control, non-transformed embryos. The expression of the BTVP2 protein was confirmed through RT-PCR (reverse transcription polymerase chain reaction) using the RNA isolated from the transgenic callus employing BTVP2- specific primers. The production of transgenic peanut was mainly focused on evaluating a newly improved somatic embryogenesis regeneration system as well as the gene transfer method and to produce the Bluetongue outer coat protein that comprises the neutralizing epitopes.
LA:	Language English
SL:	Summary Language English
PY:	Publication Year 2006
PT:	Publication Type Journal Article
PB:	Publisher Elsevier Science Ltd., The Boulevard Langford Lane Kidlington Oxford OX5 1GB UK, nlinfo- f@elsevier.nl, http://www.elsevier.nl
DO:	DOI 10.1016/j.vaccine.2005.11.019
CL:	Classification W2 32065 Plants; V 22098 Immunization: Vaccines & vaccination: Animal; F 06700 Monoclonal antibodies, hybridoma, antigens
UD:	Update 200604
SF:	Subfile Virology & AIDS Abstracts; Agricultural and Environmental Biotechnology Abstracts; Immunology Abstracts
AN:	Accession Number 6719706
PG:	Journal Pages 2994-3000
JV:	Journal Volume 24
JI:	Journal Issue 15

The following field codes are found in the records of this database. Here they are listed in alphabetical order by two-letter code.
 

AB = Abstract	IS = ISSN
AF = Author Affiliation	LA = Language
AN = Accession Number	NT = Notes
AU = Authors	NU = Other Numbers
CA = Corporate Author	OT = Original Title
CF = Conference	PB = Publisher
CL = Classification Code	PT = Publication Type
DE = Descriptors	PY = Publication Year
ED = Editor	SF = Subfile Name
EM = Entry Month	SL = Summary Language
ER = Environmental Regime	SO = Source
IB = ISBN	TI = Title
ID = Identifiers	TR = ASFA Input Center Number