Human Genome Abstracts

Human genome research is opening up a new era in which specialists will increasingly turn to molecular biology and genetics to answer long-standing scientific questions. Human Genome Abstracts offers a comprehensive source for developments and promising applications emerging from international genome research, including the massive Human Genome Project.

Subject Coverage

Major areas of coverage include:

• Nucleic acid
• Cloning vectors
• Gene cloning
• Sequencing
• Gene therapy
• Immunogenetics
• RFLP
• Mapping
• Mutagenesis
• Medical genetics
• Genetic screening

1981-1995

Over 22,395 records as of April 2006

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TI:	Title Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1
AU:	Author Eklund, EA; Lee, Seung-won; Skalnik, DG*
AF:	Author Affiliation Herman B Wells Cent. Pediatr. Res., Rm. 2600, Riley Hosp. Children, 702 Barnhill Dr., Indianapolis, IN 46202-5225, USA
SO:	Source Gene, vol. 155, no. 2, pp. 231-235, 1995
IS:	ISSN 0378-1119
AB:	Abstract We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain. This clone was obtained by ligand-screening a lambda gt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA. Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.
LA:	Language English
SL:	Summary Language English
PY:	Publication Year 1995
PT:	Publication Type Journal Article
DE:	Descriptors man; ribosomal protein S1; DNA-binding protein; cloning
CL:	Classification N 14414 Structure and sequence; G3 12780 Others
SF:	Subfile Biochemistry Abstracts 2: Nucleic Acids; Human Genome Abstracts
AN:	Accession Number 3755417

The following field codes are found in the records of this database. Here they are listed in alphabetical order by two-letter code. 

AB = Abstract	LA = Language
AF = Author Affiliation	NT = Notes
AN = Accession Number	NU = Other Numbers
AU = Authors	OT = Original Title
CA = Corporate Author	PB = Publisher
CF = Conference	PT = Publication Type
CL = Classification Code	PY = Publication Year
DE = Descriptors	SF = Subfile Name
ED = Editor	SL = Summary Language
ER = Environmental Regime	SO = Source
IB = ISBN	TI = Title
ID = Identifiers	TR = ASFA Input Center Number
IS = ISSN