Nucleic Acids Abstracts

Readers will find detailed perspectives on innovative cloning and sequencing strategies as well as gene regulatory sequences and proteins, RNA processing and transport, RNA editing DNA biosynthesis, transcription factors and oncoproteins. Other coverage includes RNA - biosynthesis, virus and phage infections, effect of hormones, role as a messenger, use of antisense methodology (oligonucleotides and RNA), and more DNA - effects of radiation, antibiotics and other agents, biosynthesis, virus and phage infections, cloning and sequencing, mutagenesis, and much more Enzymes - Ribozymes, RNases, DNases, polymerases, phosphorylases, DNA unwinding enzymes, methylguanine-DNA methyltransferase - and more.
 

Major areas of coverage include:

• Purines, Pyrimidines, and Analogs
• Nucleosides and Analogs
• Nucleotides, Nucleoside Di- and Triphosphates, and Analogs
• Oligonucleotides, Polynucleotides, and Analogs
• Transfer RNA
• rRNA and Ribosomal Proteins
• Protein Biosynthesis
• mRNA
• snRNA, snoRNA and ribonucleoproteins
• DNA
• Gene Regulation
• Gene Manipulation
• Enzymes
• Immunological Aspects
• Protein-Nucleic Acid Association

1982 - current

Monthly, with approximately 1000 new records added

Over 269,710 records as of February 2007

Nucleic Acids Abstracts (1989 - 2003)

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TI:	Title In vitro photooxidation of nucleic acids by ultraviolet A radiation
AU:	Author Wamer, WG; Wei, Rong Rong
AF:	Author Affiliation Food and Drug Admin., HFS- 128, 200 C St., S.W., Washington, DC 20204, USA
SO:	Source PHOTOCHEM. PHOTOBIOL., vol. 65, no. 3, pp. 560-563, 1997
IS:	ISSN 0031-8655
AB:	Abstract Although ultraviolet A radiation (UVA, 315-400 nm) has been shown to induce oxidative stress in mammalian cells and skin, the critical chromophore(s) and molecular target(s) involved have not been identified. We examined the role of oxidative damage to nucleic acids induced by UVA. To assess photooxidation of cellular DNA and RNA by UVA, we irradiated human skin fibroblasts with up to 765 kJ/m super(2) UVA. Cellular DNA and RNA were isolated immediately and enzymatically hydrolyzed to nucleosides. 8-Oxo-7,8- dihydro-2'-deoxyguanosine (8-oxodG), a primary oxidation product in DNA, and 8-oxo-7,8- dihydroguanosine (8-oxoG), resulting from hydroxylation of guanine in RNA, were measured by HPLC with electrochemical detection. We determined that irradiation of skin fibroblasts with levels of UVA that produced moderate toxicity also resulted in significant levels of guanine hydroxylation in RNA. Lower levels of photooxidation were observed in DNA. These results suggest that measurement of guanine hydroxylation in nucleic acids, particularly in cellular RNA, may be a powerful tool for investigating the photobiological activity of UVA.
LA:	Language English
SL:	Summary Language English
PY:	Publication Year 1997
PT:	Publication Type Journal Article
DE:	Descriptors U.V. radiation; DNA; RNA; 8-oxo- 7,8-dihydro-2'-deoxyguanosine; 8-oxo-7,8-dihydroguanosine; guanine; chromophores
CL:	Classification N 14630 Chemical reactions & interactions, including effects of radiation
EM:	Entry Month 9705
SF:	Subfile Biochemistry Abstracts 2: Nucleic Acids
AN:	Accession Number 4039552

The following field codes are found in the records of this database. Here they are listed in alphabetical order by two-letter code.

AB = Abstract	IS = ISSN
AF = Author Affiliation	LA = Language
AN = Accession Number	NT = Notes
AU = Authors	NU = Other Numbers
CA = Corporate Author	OT = Original Title
CF = Conference	PB = Publisher
CL = Classification	PT = Publication Type
DE = Descriptors	PY = Publication Year
ED = Editor	SF = Subfile
EM = Entry Month	SL = Summary Language
ER = Environmental Regime	SO = Source
IB = ISBN	SO = Source
ID = Identifiers	TR = ASFA Input Center Number