| TI:
|
Title
The inner-nuclear-envelope protein emerin
regulates HIV-1 infectivity |
| AU:
|
Author
Jacque, Jean-Marc; Stevenson, Mario |
| AF:
|
Author Affiliation
Program in Molecular Medicine, University of
Massachusetts Medical School , 373 Plantation
Street, Suite 319, Worcester, Massachusetts 01605,
USA |
| SO:
|
Source
Nature [Nature]. Vol. 441, no. 7093, pp. 641-645.
Jun 2006 |
| IS:
|
ISSN
0028-0836 |
| DE:
|
Descriptors
Infection; Macrophages; cDNA; Chromatin; Nuclear
membranes; emerin; HIV-1; Human immunodeficiency
virus 1 |
| AB:
|
Abstract
Primate lentiviruses such as human
immunodeficiency type 1 (HIV-1) have the capacity
to infect non-dividing cells such as tissue
macrophages. In the process, viral complementary
DNA traverses the nuclear envelope to integrate
within chromatin. Given the intimate association
between chromatin and the nuclear envelope, we
examined whether HIV-1 appropriates nuclear
envelope components during infection. Here we show
that emerin, an integral inner-nuclear-envelope
protein, is necessary for HIV-1 infection.
Infection of primary macrophages lacking emerin
was abortive in that viral cDNA localized to the
nucleus but integration into chromatin was
inefficient, and conversion of viral cDNA to
non-functional episomal cDNA increased. HIV-1 cDNA
associated with emerin @@iin vivo@, and the
interaction of viral cDNA with chromatin was
dependent on emerin. Barrier-to-autointegration
factor (BAF), the LEM (LAP, emerin, MAN) binding
partner of emerin, was required for the
association of viral cDNA with emerin and for the
ability of emerin to support virus infection.
Therefore emerin, which bridges the interface
between the inner nuclear envelope and chromatin,
may be necessary for chromatin engagement by viral
cDNA before integration. |
| LA:
|
Language
English |
| SL:
|
Summary Language
English |
| PY:
|
Publication Year
2006 |
| PD:
|
Publication Date
200606 |
| PT:
|
Publication Type
Journal Article |
| PB:
|
Publisher
Nature Publishing Group, The Macmillan Building, 4
Crinan Street, London, N1 9XW, UK; |
| DO:
|
DOI
10.1038/nature04682 |
| CL:
|
Classification
N 14025 RNA/DNA role in infection & immune
response; V 22002 AIDS: Molecular and in vitro
aspects |
| UD:
|
Update
200607 |
| SF:
|
Subfile
Biochemistry Abstracts 2: Nucleic Acids; Virology
& AIDS Abstracts |
| AN:
|
Accession Number
6854954 |
| PG:
|
Journal Pages
641-645 |
| JV:
|
Journal Volume
441 |
| JI:
|
Journal Issue
7093 |
